Enzymatic colorimetric method for the determination of inorganic phosphorus in serum and urine.

Determination of inorganic phosphorus in serum: Evaluation of three methods applied to the Technicon RA-1000 analyzer

We have evaluated three analytical methods for determining inorganic phosphorus in serum applied to the Technicon RA-I000 analyzer: a fully enzymatic colorimetric method based on the specific system purine nucleoside phosphorylase/xanthine oxidase coupled to an indicator colorimetric reaction similar to the Trinder reaction; a chemical method involving the direct UV measurement of the phosphomo...

A method for the colorimetric determination of calcium and magnesium in small amounts of urine, stool, and food.

This report presents a method for the quantitative determination of magnesium and calcium in a single specimen of urine, stool, and food by formation and calorimetric determination of their respective phosphates. The method makes use of some of the principles advanced by Roe and Kahn (1) for determination of serum calcium, and by Denis (2) for serum magnesium. Phosphorus determinations are perf...

New Enzymatic Colorimetric Method for the Quantitative Determination of Phenylalanine in Dry-Blood Spots

Phenylketonuria (PKU) is an autosomal recessive disorder, which is characterized by severe mental retardation, microcephaly and seizures. The symptoms of this disease can be prevented if detected soon after birth. Therefore, blood Phenylalanine (Phe) measurement is essential for the early diagnosis, treatment and dietary monitoring of PKU patients. The goal of this research was to introduce a r...

Colorimetric vs enzymatic determination of serum phosphorus.

Urinary inorganic phosphorus determinations.

The Parekh-Jung method for determination of inorganic phosphorus in serum was applied to its determination in urine. Accuracy is good. The mean percentage recovery of added phosphorus was 100.2%. Forty analyses of a sample gave a mean value of 46.71 mg/100 ml +/- 0.76 standard deviation (between-batch precision). The relative usefulness of the Parekh-Jung method compared with those other method...